Analysis of the Expression and Polymorphism of APOE, HSP, BDNF, and GRIN2B Genes Associated with the Neurodegeneration Process in the Pathogenesis of Primary Open Angle Glaucoma

Table 2

Primer sequences and restriction endonucleases used in the gene polymorphisms analysis by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).

Gene polymorphism	Primer sequence	Annealing; °C	PCR; product bp	Enzyme
rs1043618	F: 5′-CGCCATGGAGACCAACACCC-3′; R: 5′-GCGGTTCCCTGCTCTCTGTC-3′	63	488	BsrBI
rs3764028	F: 5′-CGCTCTCCGTCGGTGCTGTT-3′; R: 5′-CTGGGGAAGTGGGGTGGTAACG-3′	61	115	HpyCH4IV
rs2030324	F: 5′-TTGCACATCCTGCTCAAGTC-3′; R: 5′-TTGCTAGGAGAAAAGCCATGA-3′	62	348	HpyCH4IV
rs449647	F: 5′-CAAGGTCACACAGCTGGCAAC-3′; R: 5′-TCCAATCGACGGCTAGCTACC-3′	69	1426	—
	F (mismatched): 5′-TGTTGGCCAGGCTGGTTTTAA-3′; R: 5′-CTTCCTTTCCTGACCCTGTCC-3′	63	227	DraI