Analysis of the Expression and Polymorphism of APOE, HSP, BDNF, and GRIN2B Genes Associated with the Neurodegeneration Process in the Pathogenesis of Primary Open Angle Glaucoma

Table 3

Primer sequences used in QRT-PCR analysis.

Gene	Primer sequence	PCR; product [bp]
GAPDH	F: 5′-CACCTTCCCCATGGTGTCT-3′; R: 5′-CCCCGGTTTCTATAAATTGAGC-3′	120
BDNF	F: 5′-CAAATACAAGACCCTGCTTGTG-3′; R: 5′-CCACCATTTTTGCACTTGCTT-3′	90
APOE	F: 5′-TGGACAAGTCTGGGATCCTT-3′; R: 5′-CATCTTCCTGCCTGTGATTG-3′	78
HSP70-1	F: 5′-GGGAAGCCTTGGGACAAC-3′; R: 5′-TGATTGGCTCAGAAGGGAAA-3′	80
GRIN2B	F: 5′-AGCAATGGGACTGTCTCACC-3′; R: 5′-AACATCATCACCCATACGTCAG-3′	85