A MicroRNA-Transcription Factor Blueprint for Early Atrial Arrhythmogenic Remodeling

Figure 4

Altered expression of Pitx2, Tbx5, and Myocd in response to atrial short-term tachyarrhythmia. (a) Average relative values of transcript levels of Pitx2c, Tbx5, and Myocd in the left atrium from paced (EXP) versus sham (CON) animals. ( for each group). (b) Representative Western blots of left atrium samples from sham-operated (CON, lines 1-2) and paced (EXP, lines 3 and 4) animals and overall relative levels of the proteins as based on average values from each group. ( for each group). Western blot replicates were probed with antibodies against PITX2A,B,C, TBX5, MYOCD (myocardin), CASP3 (caspase-3), CASP9 (caspase-9), SERCA2 (cardiac sarcoplasmic reticulum Ca(2+)-ATPase 2a), CASQ2 (cardiac calsequestrin), TNNI3 (cardiac troponin I), and GAPDH (glyceraldehyde-3-phosphate dehydrogenase). MW values of the bands detected are shown.

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