Role of Baicalin in Anti-Influenza Virus A as a Potent Inducer of IFN-Gamma

Figure 6

Effects of BA in JAK/STAT-1 pathway. Human PBMC were treated with or without 1 mM BA for 24 h, 48 h, and 72 h at 37°C. (a) The cytosol protein was separated by electrophoresis in a 12.5% SDS-polyacrylamide gel and transferred to a nitrocellulose membrane. The proteins were blotted with anti-JAK-1, anti-JAK-2, anti-phosphotyrosine 4G10, and anti-phospho-JAK-2-Y1007/Y1008 Abs, respectively. (b) The nucleus proteins (upper panels) and cytosol proteins (lower panels) were obtained and analyzed by Western Blotting using Abs against STAT-1, phosphor-STAT-1 (tyrosine 701), and phosphor-STAT-1 (serine 727).

(a)

(b)