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BioMed Research International
Volume 2015, Article ID 267538, 5 pages
Research Article

Improved Sugar Production by Optimizing Planetary Mill Pretreatment and Enzyme Hydrolysis Process

1Korea Institute of Ceramic Engineering and Technology (KICET), 101 Soho-ro, Jinju-si, Gyeongsangnam-do 52851, Republic of Korea
2Department of Chemical Engineer, Hanyang University, Seoul 04763, Republic of Korea
3School of Nano-Bioscience and Chemical Engineering, Ulsan National Institute of Science and Technology, Ulsan 44919, Republic of Korea
4Department of Forest Products and Technology, Chonnam National University, Gwangju 61186, Republic of Korea

Received 19 May 2015; Revised 30 July 2015; Accepted 9 August 2015

Academic Editor: Yu Zhang

Copyright © 2015 Jeong Heo Kwon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper describes an optimization of planetary mill pretreatment and saccharification processes for improving biosugar production. Pitch pine (Pinus rigida) wood sawdust waste was used as biomass feedstock and the process parameters optimized in this study were the buffering media, the milling time, the enzyme quantity, and the incubation time. Glucose yields were improved when acetate buffer was used rather than citrate buffer. Initially, with each process variable tests, the optimal values were 100 minutes of milling, an enzyme concentration of 16 FPU/g-biomass, and a 12-hour enzymatic hydrolysis. Typically, interactions between these experimental conditions and their effects on glucose production were next investigated using RSM. Glucose yields from the Pinus rigida waste exceeded 80% with several of the conditions tested, demonstrating that milling can be used to obtain high levels of glucose bioconversion from woody biomass for biorefinery purposes.