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BioMed Research International
Volume 2015, Article ID 267989, 8 pages
http://dx.doi.org/10.1155/2015/267989
Research Article

Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer’s Disease

1Laboratório de Genética, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Rua Acre s/n, Bloco 2E sala 230, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil
2Faculdade de Medicina, Universidade Federal de Uberlândia, Avenida Para 1720, Bloco 2U sala 23, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil
3Laboratório de Nanobiotecnologia, Instituto de Genética e Bioquímica, Universidade Federal de Uberlândia, Rua Acre s/n, Bloco 2E sala 230, Campus Umuarama, 38400-902 Uberlândia, MG, Brazil

Received 28 June 2014; Accepted 18 February 2015

Academic Editor: Giovanni Scapagnini

Copyright © 2015 Luiz Carlos de Oliveira-Júnior et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Autoantibodies (aAb) associated with Alzheimer’s disease (AD) have not been sufficiently characterized and their exact involvement is undefined. The use of information technology and computerized analysis with phage display technology was used, in the present research, to map the epitope of putative self-antigens in AD patients. A 12-mer random peptide library, displayed on M13 phages, was screened using IgG from AD patients with two repetitions. Seventy-one peptides were isolated; however, only 10 were positive using the Elisa assay technique (Elisa Index > 1). The results showed that the epitope regions of the immunoreactive peptides, identified by phage display analysis, were on the exposed surfaces of the proteins. The putative antigens MAST1, Enah, MAO-A, X11/MINT1, HGF, SNX14, ARHGAP 11A, APC, and CENTG3, which have been associated with AD or have functions in neural tissue, may indicate possible therapeutic targets.