(a)
(b)
Figure 3: (a) Quantification of apoptotic human PBMCs by TUNEL assay after treatment with hot aqueous extract of Achyrocline satureioides (10, 600, and 1000 μg/mL). (b) Photomicrographs of human PBMCs treated with hot aqueous extract of Achyrocline satureioides (As-HAE) and stained with TUNEL (100x) using the commercial kit ApopTag Plus Peroxidase In Situ Apoptosis (Chemicon International, USA). (A) Medium alone (control), (B) cells treated with hydrogen peroxide (1 mmol/L), (C) As-HAE (10 μg/mL), (D) As-HAE (600 μg/mL), and (E) As-HAE (1000 μg/mL). Apoptotic cells are shown in brown.