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BioMed Research International
Volume 2015 (2015), Article ID 270973, 12 pages
Research Article

In Vitro and In Vivo Cytogenotoxic Effects of Hot Aqueous Extract of Achyrocline satureioides (Lam.) DC.

1Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, Río Cuarto, C5800 Córdoba, Argentina
2Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Rivadavia 1917, C1033AAJ Buenos Aires, Argentina
3Área de Microscopía Electrónica, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, Río Cuarto, C5800 Córdoba, Argentina
4Farmacognosia, Departamento de Farmacia, Universidad Nacional de Córdoba (IMBIV-CONICET), Ciudad Universitaria, C5000 Córdoba, Argentina

Received 26 December 2014; Revised 1 February 2015; Accepted 1 February 2015

Academic Editor: Isabel C. F. R. Ferreira

Copyright © 2015 L. N. Cariddi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The identification of flavonoids in an enriched fraction (ethyl ether) from the hot aqueous extract of Achyrocline satureioides (Lam.) DC (As-HAE) is shown below. By means of HPLC-ESI-MS/MS analysis we identified quercetin, luteolin and as 3-O-methylquercetin in this extract.

Fig. 1 shows the identification of quercetin (tR= 18.3) in the extract because its peak [M-1]= 301 was detected with its characteristic MS/MS rupture, which all are coincident with the data obtained for standard quercetin (Fig. 2). Fig. 3 shows the peak [M-1]= 285 corresponding to luteolin in the extract (tR= 21.6), which did not suffer rupture as the standard luteolin (Fig. 4). In Fig. 5 we can observe the peak [M-1]= 315 corresponding to 3-O-methylquercetin (tR= 21.3), which has a MS/MS rupture according to this structure since the loss of methyl was observed to give the peak 300 m/z (quercetin).

  1. Supplementary Material