Candesartan Mediated Amelioration of Cisplatin-Induced Testicular Damage Is Associated with Alterations in Expression Patterns of Nephrin and Podocin

Figure 2

Changes in expression levels of nephrin and podocin in the testes of rats following treatment with cisplatin and/or candesartan. (a) Total RNA was extracted from the testes and analyzed for nephrin mRNA levels by real-time RT-PCR. Each column represents the mean value with standard deviation. ^†, , and differ from Group 1, Group 3, and Group 5, respectively (). (b) Total RNA was extracted from the testes and analyzed for podocin mRNA levels by real-time RT-PCR. Each column represents the mean value with standard deviation. ^†, , and are represented as in (a). (c) Protein was extracted from the testes, and expression levels of nephrin, podocin, and β-actin were analyzed by Western blotting. (d) Nephrin and β-actin signal intensity in (c) were quantified, and the ratio of nephrin over β-actin (d) and that of podocin over β-actin (e) were presented in histogram format. ^†, , and are represented as in (a).

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