Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell

Figure 3

Split-GFP assay to monitor VirE2 import into yeast cells during AMT process. (a) The VirE2 transport rate and the number of VirE2 aggregates in each cell increase during the 24-hour time course in both WT and arp6Δ mutant. (b) The import rates were similar for both strains at the beginning, but the percentage was much higher in the mutant after 21 h. The vector pQH04 in the yeast cells contains the large fragment of GFP. Error bars present for SD. by -test.

(a)

(b)