Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell

Figure 4

Effects of VirD2 degradation in WT and arp6Δ on AMT efficiency. (a) Time course experiment for VirD2-GFP fusion protein and GFP degradation in WT and arp6Δ after inhibition of new protein synthesis. The yeast cells were cultivated in SD ura-galactose medium overnight before addition of cycloheximide to the final concentration of 10 μg/mL. (b) The effect of VirD2 induction on AMT efficiency. The results were averaged from three independent experiments. Error bars present for SD. by -test.

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