Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell

Figure 5

The disrupted microtubule network in arp6Δ was beneficial for AMT process. (a) The loss of ARP6 resulted in disrupted microtubule structure which is similar to the effect of colchicine/oryzalin treatment. (b) The effect of colchicine and oryzalin on AMT efficiency. 5 × 10⁵ yeast cells and 10⁸ bacteria were cocultivated at 20°C for 24 h. The results were the average of three independent experiments. Error bars present for SD. by -test.

(a)

(b)