Yeast Actin-Related Protein ARP6 Negatively Regulates Agrobacterium-Mediated Transformation of Yeast Cell

Figure 6

ARP6 affects stability but not the uptake of T-DNA. (a) FISH results for cocultivation mixture of yeast and Agrobacterium for 24 h. T-DNA at the periphery of yeast cell and in the nucleus of the yeast cell was detected by Cy3-labeling probes. DAPI staining indicates the location of yeast nuclei. The arrows indicate the probes hybridized to T-DNA. (b) Nuclease activity assay for WT and arp6Δ lysates. Lanes 1–3: arp6Δ lysates 10, 20, and 30 μL, respectively; lanes 4–6: WT lysates 10, 20, and 30 μL, respectively; lane 7: undigested plasmid DNA. (c) Normalized data for the activity of DNase analyzed by Image J. Error bars present for SD. by -test.

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