Antimicrobial Resistance Profile and Genotypic Characteristics of Streptococcus suis Capsular Type 2 Isolated from Clinical Carrier Sows and Diseased Pigs in China

Table 1

Target genes and PCR primers used in this study.

Gene target(s)	Primer sequence (5′-3′)	Amplicon size (bp)	Reference
Macrolide/Lincosamide resistance genes
ermB	GAAAAGGTACTCAACCAAATA	639	[29]
	AGTAACGGTACTTAAATTGTTTAC
ermA	GAAGTTTAGCTTTCCTAA	395	[29]
	GCTTCAGCACCTGTCTTAATTGAT
mefA	AGTATCATTAATCACTAGTGC	346	[29]
	TTCTTCTGGTACTAAAAGTGG
lnuB	CCTACCTATTGTTTGTGGAA	944	[29]
	ATAACGTTACTCTCCTATTC
Tetracycline resistance genes
tetK	TATTTTGGCTTTGTATTCTTTCAT	1159	[26]
	GCTATACCTGTTCCCTCTGATAA
tetL	ATAAATTGTTTCGGGTCGGTAAT	1077	[26]
	AACCAGCCAACTAATGACAATGAT
tetO	AACTTAGGCATTCTGGCTCAC	519	[26]
	TCCCACTGTTCCATATCGTCA
tetM	GAACTCGAACAAGAGGAAAGC	740	[26]
	ATGGAAGCCCAGAAAGGAT
IntTn and xis genes
intTn	GGTCTTCGTATTTCAGAGTTTGG	473	[30]
	GTTGCATGTGCGTAATAGTTCAG
xis	AAGCAGACTGACATTCCTA	193	[30]
	GCGTCCAATGTATCTATAA
Virulence-associated factors
mrp	ATTGCTCCACAAGAGGATGG	188	[15]
	TGAGCTTTACCTGAAGCGGT
epf	CGCAGACAACGAAAGATTGA	744	[15]
	AAGAATGTCTTTGGCGATGG
sly	GCTTGACTTACGAGCCACAA	248	[15]
	CCGCGCAATACTGATAAGC
fbp	GACGGATCCTTTTTACATCACATGACGG	247	this study
	CCGTCGACGTATTTCCGCAGAATCAT
orf2	CAAGTGTATGTGGATGGG	860	this study
	ATCCAGTTGACACGTGCA
gdh	GGCGCCGAATTCGTCGACATTTAGCAATTTTTGCG	1039	this study
	CGCCGCGGATCCGTAGTTAAAGTTGGTATTAAC