The Histone Acetyltransferase Gcn5 Regulates ncRNA-ICR1 and FLO11 Expression during Pseudohyphal Development in Saccharomyces cerevisiae

Figure 2

The histone acetyltransferase activity of Gcn5 is required for pseudohyphal development. (a) The histone acetyltransferase activity of Gcn5 is required for haploid invasive growth. The gcn5 deletion haploid (gcn5 + vector) strain carrying a wild type GCN5 (gcn5 + GCN5) or a catalytic dead Gcn5 (gcn5 + E173Q) was grown on YPD agar plate for 4 d at 30°C before invasive growth was scored. The flo11 was used as a control for complete loss of invasive growth. The quantification of invasiveness in each strain was measured by cells remaining on agar before (total growth) and after being washed (invasive growth) and is shown on the right. Data are mean ± SD from 5 measurements in 10 biological repeats. (b) Histone acetyltransferase activity of Gcn5 is required for diploid filamentous growth. The indicated diploid strains were grown on nitrogen starvation agar medium (SLAD) at 30°C and the filamentous growth of colonies was recorded daily for 6 d. The quantification of filament formation was described in Section 2. Data are mean ± SD from measurements of filamentous colonies in 10 biological repeats. Scale bar: 0.2 mm.

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