Testosterone Depletion Induces Demethylation of Murine Reelin Promoter CpG Dinucleotides: A Preliminary Study

Figure 1

(a) Murine Reln CpG island prediction and MSP primer design. Blue regions delimited on mouse reelin gene promoter sequence (first 1200 bases) indicate CpG islands predicted by MethPrimer software (http://www.urogene.org/methprimer/). General parameters for CG-rich regions are depicted in Section 2.4. The small red lines indicate C residues from CpG dinucleotides alongside Reln promoter region. Green boxes indicate annealing regions for MSP forward (UF3) and reverse (UR3) primers. (b) Alignment of segments of DNA sequences from reelin promoter regions of different species showing the conserved cytosine at position −860.

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