Identification of Extended-Spectrum β-Lactamases Escherichia coli Strains Isolated from Market Garden Products and Irrigation Water in Benin

Table 1

Primers used to search genes in this study.

	Target genes	Primers	Primers sequences (5′ → 3′)	Amplicon size (bp)	Reference
E. coli multirestant		OT-F; OT-R	5′-TTGGGTGCACGAGTGGGTTA-3′; 5′-TAATTGTTGCCGGGAAGCTA-3′	467	[13]
E. coli multirestant		SHV-F; SHV-R	5′-CGCCGGGTTATTCTTATTTGTCGC-3′; 5′-TCTTTCCGATGCCGCCGCCAGTCA-3′	1017	[14]
E. coli multirestant		CTX-F; CTX-R	5′-CGCTTTGCGATGTGCAG-3′; 5′-ACCGCGATATCGTTGGT-3′	550	[13]
EHEC	VT	VT-F; VT-R	5′-GAGCGAAATAATTTATATGTG-3′; 5′-TGATGATGGCAATTCAGTAT-3′	518	[15]
STEC	SLTI (Stx1)	SLTI-F; SLTI-R	5′-GAAGAGTCCGTGGGATTACG-3′; 5′-AGCGATGCAGCTATTAATAA-3′	150	[16]
STEC	SLTII (Stx2)	SLTII-F; SLTII-R	5′-TTAACCACACCCACGGCAGT-3′; 5′-GCTCTGCATGCATCTCTGGT-3′	255	[16]
ETEC	LT	LT-F; LT-R	5′-GCGACAAATTATACCGTGCT-3′; 5′-CCGAATTCTGTTATATATGT-3′	315	[16]

VT: verotoxin, EHEC: enterohemorrhagic E. coli, LT: heat-labile enterotoxin, ETEC: enterotoxigenic E. coli, SLTI: Shiga-like toxin I, SLTII: Shiga-like toxin II, and STEC: Shiga toxin E. coli.