|
| Strains or plasmids | Relevant genotype or features | Source or reference |
|
| Strains | | |
| XL1-Blue MRF′ | Δ mcrA183 Δ mcrCB-hsdSMR-mrr173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac proAB ZΔM15 Tn10 | Stratagene |
| Shuffle Express | fhuA2 lon ompT ahpC gal λatt::pNEB3-r1-cDsbC , ΔtrxB sulA11 R mcr-73::miniTn10– 2 dcm R zgb-210::Tn10– endA1 Δgor Δ mcrC-mrr114::IS10 | NEB1 |
| BW25113 | Δ araD-araB567 ΔlacZ4787(::rrnB-3) rph-1 Δ rhaD-rhaB568 hsdR514 | CGSC2
[54] |
| JW2861-1 | BW25113 dsbC744::kan | GCSC2
[54] |
| Plasmid | | |
| pBAD33 | Arabinose regulation, p15A origin, | ATCC3
[55] |
| pBAD-AppA | pBAD33-based, periplasmic AppA | This study |
| pBluescript SK- | Lactose regulation, ColE1 origin, | Stratagene |
| pBAppA | pBluescript-based, periplasmic AppA | This study |
| pBPelB-EhPDI | pBluescript-based, periplasmic EhPDI (wild type) | [22] |
| pBRM05 | pBluescript-based, periplasmic EhPDISS/CC (C44S; C47S) | [20] |
| pBRM06 | pBluescript-based, periplasmic EhPDICC/SS (C160S; C163S) | [20] |
| pBRM15 | pBluescript-based, periplasmic EhPDISS/SS (C44S; C47S; C160S; C163S) | [20] |
| pQE30 | Lactose regulation, ColE1 origin, | Qiagen |
| pQHPDI | pQE30-based, recombinant EhPDI (wild type) | [20] |
| pQRM05 | pQE30-based, recombinant EhPDISS/CC (C44S; C47S) | This study |
| pQRM06 | pQE30-based, recombinant EhPDICC/SS (C160S; C163S) | This study |
| pQRM15 | pQE30-based, recombinant EhPDISS/SS (C44S; C47S; C160S; C163S) | This study |
|
