Analysis of the Isomerase and Chaperone-Like Activities of an Amebic PDI (EhPDI)

Table 3

In vitro activities of purified EhPDI enzyme variants.

Enzyme	RNAse A oxidative refolding		Insulin reduction; ×10^−6 /min^2; (%)
	Oxidase; ×10^−6 min^−1 (%)	Isomerase; ×10^−6 M/min (%)
EhPDI	513 ± 16 (100)	39 ± 1 (100)	109 ± 11 (100)
EhPDISS/CC	551 ± 16 (107)	33 ± 1 (85)	22 ± 2 (20)
EhPDICC/SS	517 ± 13 (101)	31 ± 1 (79)	65 ± 5 (60)

The data was expressed as mean ± standard error ( = 3).
The activity of the enzyme variant EhPDI_SS/SS was not statistically significant, as compared with the reaction performed in the absence of enzyme.
The activity ratio (%) was calculated as (variant/wild type) × 100.