Production by Tobacco Transplastomic Plants of Recombinant Fungal and Bacterial Cell-Wall Degrading Enzymes to Be Used for Cellulosic Biomass Saccharification

Figure 1

Portion of the chloroplast transformation vector map containing the gene coding for Vlp2 (pVSR326Vlp2), integration site of tobacco chloroplast DNA (cpDNA), and the same region in transplastomic tobacco plants are shown. Also restriction map of vectors containing genes coding for other cellulolytic enzymes is shown. All the genes coding for cellulolytic enzymes were put under the expression signals of rice psbA. Lines with double arrow indicate the size of DNA fragments after the restriction digestion with ClaI restriction enzyme. Dashed arrow indicates the FLK probe (rbcL-aacD flanking region) used to confirm site specific integration of transgenes. A possible mechanism for site-specific integration of aadA and Vlp2 through two homologous recombination events (crossed lines) is also shown. Size of the coding region of each gene is shown in brackets.