Nanoparticle Labeling of Bone Marrow-Derived Rat Mesenchymal Stem Cells: Their Use in Differentiation and Tracking

Table 2

RT-PCR conditions for each primer.

Genes	Initial denaturation	Denaturation	Annealing	Extension	Cycle	Final extension
Beta-actin	95^°C, 5 min	94^°C 40 sec	60^°C 35 sec	72^°C 40 sec	25	72^°C, 5 min
CD90	95^°C, 5 min	94^°C 30 sec	55^°C 30 sec	72^°C 30 sec	30	72^°C, 5 min
CD34	95^°C, 5 min	94^°C 30 sec	55^°C 30 sec	72^°C 30 sec	30	72^°C, 5 min
CD71	95^°C, 5 min	94^°C 40 sec	66^°C 60 sec	72^°C 40 sec	35	72^°C, 5 min
CD29	95^°C, 5 min	94^°C 30 sec	60^°C 30 sec	72^°C 30 sec	29	72^°C, 5 min
CD45	95^°C, 5 min	94^°C 30 sec	60^°C 30 sec	72^°C 30 sec	23	72^°C, 5 min