Prokineticin 2 Upregulation in the Peripheral Nervous System Has a Major Role in Triggering and Maintaining Neuropathic Pain in the Chronic Constriction Injury Model

Figure 3

Time-course of PROK2 mRNA expression in injured sciatic nerve and ipsilateral DRG of CCI-saline mice and CCI-PC1 mice. PROK2 levels in the peripheral nervous system of healthy animals were negligible ( Ct in sciatic nerve, Ct in DRG). In the sciatic nerve (a) PROK2 expression was significantly increased at 3 dpi, reached its maximal expression at 10 dpi, and then started to decrease up to 17 dpi. In the ipsilateral L 4–6 DRGs (b) PROK2 mRNA was significantly increased at 6 dpi and showed a constant tendency to increase up to 17 dpi. Data are mean ± SEM of 5 animals. One-way ANOVA was used for statistical evaluation, followed by Tukey test for multiple comparisons. ^*, ^*** CCI-saline versus sham; ^° CCI-PC1 versus CCI-saline.

(a)

(b)