Figure 2: Effects of PFOS on the apoptosis of SH-SY5Y cells. (a and b) Cells were treated with DMSO (vehicle control) or PFOS (10, 50, and 100 μM) for 48 h. Nuclear morphology was monitored by fluorescent inverted microscopy after staining the cells with AO/EB fluorescent dyes. Magnification, 100x. Six hundred cells were analysed, and the number of apoptotic cells was expressed as a percentage of the total number of analysed cells. L: live cells; Ap: apoptotic cells; N: necrotic cells. Data are presented as mean ± SD of three independent experiments. , experimental values compared with control values.