Efficient Mitochondrial Genome Editing by CRISPR/Cas9

Table 2

Real-time PCR primers.

Gene	Forward	Reverse
Cox1	CGCCGACCGTTGACTATTCT	GGGGGCACCGATTATTAGGG
Cox2	TTCATGATCACGCCCTCATA	TAAAGGATGCGTAGGGATGG
Cox3	CAGCCCATGACCCCTAACAG	TGTGGTGGCCTTGGTATGTG
ND1	TCTCACCATCGCTCTTCTAC	TTGGTCTCTGCTAGTGTGGA
GAPDH	CATGTGCAAGGCCGGCTTCG	CTGGGTCATCTTCTCGCGGT
GAPDH for cDNA	AAACCCATCACCATCTTCCAG	AGGGGCCATCCACAGTCTTCT

Forward and reverse primers are designed to anneal upstream and downstream regions flanking gRNA target sites for each mtDNA locus or specific genes.