Human Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated Vγ9Vδ2 T Lymphocytes In Vitro

Figure 2

UC-MSCs inhibit the proliferation of γδ T cells in a cell-cell contact-independent manner. PBMCs from three healthy donors were labeled with CSFE and cocultured with, or without, UC-MSCs at the different ratios in transwell or together in 6-well plates, followed by simulation with PAM and IL-2 for 14 days. Subsequently, the cells were stained with fluorescent antibodies, as described in the method section. The cells were gated on CD3+TCRγδ+ and the percentages of proliferative γδ T cells were determined by flow cytometry. Data are expressed as the mean percentages ± SEM of each group of cells from three separate experiments. (a) The percentages of proliferative γδ T cells following a separated coculture in transwell plates. (b) The percentages of proliferative γδ T cells following coculture in transwell or 24-well plates. versus the controls. UC-MSC: umbilical cord mesenchymal stem cell; PBMC: peripheral blood mononuclear cell.

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