Human Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated Vγ9Vδ2 T Lymphocytes In Vitro

Figure 3

UC-MSCs modulate the expression of cytokines and bioactive effectors in γδ T cells. PBMCs were isolated and stimulated with PAM and IL-12 for 12 days. The enriched γδ T cells were cocultured with UC-MSCs at the indicated ratios for 48 hours and the cells were stained with different fluorescent antibodies, as described in in the method section. Subsequently, the percentages of IFNγ+, granzyme B+, TNFα+, perforin+, or IL-10+ γδ T cells were characterized by flow cytometry. Data are representative flow cytometry charts or expressed as the mean percentages ± SEM of each group of cells from nine subjects of nine separate experiments. (a–e) Quantitative analysis of the percentages of γδ T cells. versus the controls. UC-MSC: umbilical cord mesenchymal stem cell; PBMC: peripheral blood mononuclear cell; TNFα: tumor necrosis factor; IFNγ: interferon-γ; IL-10: interleukin-10.

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