Human Umbilical Cord Mesenchymal Stem Cells Inhibit the Function of Allogeneic Activated Vγ9Vδ2 T Lymphocytes In Vitro

Figure 5

UC-MSCs modulate the expression of Fas-L and TRAIL on γδ T cells but do not affect the spontaneous apoptosis of activated γδ T cells. PBMCs were cocultured with, or without, the different ratios of UC-MSCs in the presence of PAM and IL-2 for 14 days. The cells were stained with fluorescent antibodies, as described in in the method section. The percentages of apoptotic γδ T cells and Fas-L+ or TRAIL+ γδ T cells were characterized by flow cytometry analysis. Data are representative flow cytometry analysis of the frequency of apoptotic γδ T cells or expressed as the mean percent ± SEM of each group of cells from 19 subjects. (a–c) The quantitative analysis of apoptosis, Fas-L, and TRAIL. , versus the controls. UC-MSC: umbilical cord mesenchymal stem cell; PBMC: peripheral blood mononuclear cell; Fas-L: Fas ligand; Trail: tumor necrosis factor-related apoptosis-inducing ligand.

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