Single Nucleotide Polymorphisms of the GJB2 and GJB6 Genes Are Associated with Autosomal Recessive Nonsyndromic Hearing Loss

Table 2

Validation methods for each SNP analyzed.

SNP	Gene	Method (enzyme)
rs3751385	GJB2	RFLP-PCR (Nhe I)
rs7994748		RFLP-PCR (Bstx I)
rs7329857		RFLP-PCR (Dpn II)
rs7987302		Direct sequencing
rs7322538	GJB6	Direct sequencing
rs9315400		RFLP-PCR (Acu I)
rs877098		RFLP-PCR (Eco RI)
rs945369		RFLP-PCR (Mva I)
rs7333214		RFLP-PCR (Mae II)