Prohibitin: A Novel Molecular Player in KDEL Receptor Signalling

Figure 1

The KDELR–PHB interaction. (a) PHB coimmunoprecipitates with the KDELR. Protein from wild-type (HeLa-wt, control) and stably transfected KDELR-myc (HeLa-myc) HeLa cells were immunoprecipitated using anti-myc antibodies. The proteins from cell lysates (Total lysates) and immunoprecipitated (IPs) were separated by polyacrylamide gel electrophoresis and analysed by Western blotting for PHB and KDELR-myc. The images shown are representative of three independent experiments. (b) BFA treatment dissociates the KDELR–PHB complex. HeLa-myc cells were treated with vehicle (Control) or for 5 min with 5 μg/mL BFA and the proteins were immunoprecipitated with anti-myc antibodies. The proteins from cell lysates (Total lysates) and immunoprecipitated (IPs) were separated by polyacrylamide gel electrophoresis and analysed by Western blotting for PHB and KDELR-myc, as indicated. The images shown are representative of two independent experiments.

(a)

(b)