Prohibitin: A Novel Molecular Player in KDEL Receptor Signalling

Figure 2

Intracellular distribution of PHB. (a) PHB mainly localises to the mitochondria. COS-7 cells were fixed, permeabilised, and stained for PHB (green) and Mito-Tracker (red). The merged image of green and red signals is also shown. (b) Colocalisation analysis of PHB and Golgi proteins. COS-7 cells were transiently transfected with GFP-tagged PHB and myc-tagged KDELR. The day after, the cells were fixed and stained for myc (red), and GM130 (blue). The merged image of the green (PHB-GFP), red and blue signals is also shown. (a, b) The images shown are representative of three independent experiments. Scale bars, 10 μm. (c) Quantification of PHB-GFP fluorescence colocalizing with GM130. The black bar indicates the extent of GM130 overlapping with PHB. The gray bar indicates the extent of PHB overlapping with GM130. Data are means of overlapping coefficient ± SEM, representative of two independent experiments assessing at least 25 cells each. AU: arbitrary units.

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