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BioMed Research International
Volume 2015, Article ID 319454, 13 pages
Research Article

Prohibitin: A Novel Molecular Player in KDEL Receptor Signalling

1Department of Cellular and Translational Pharmacology, Fondazione Mario Negri Sud, 66030 Santa Maria Imbaro, Italy
2Department of Cell Biology & Signalling, The FIRC Institute of Molecular Oncology (IFOM), 20139 Milan, Italy
3Proteomic Core Facility, Fondazione Mario Negri Sud, 66030 Santa Maria Imbaro, Italy
4Institute of Protein Biochemistry, National Research Council and Telethon Institute of Genetics and Medicine, 80131 Naples, Italy

Received 5 December 2014; Accepted 14 April 2015

Academic Editor: Christian Appenzeller-Herzog

Copyright © 2015 Monica Giannotta et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Characterization of HeLa-myc cells.

Supplementary Fig S1. shows that the intracellular distribution of myc-tagged KDELR stably transfected in Hela cells (HeLa-myc cells) was similar to that of endogenous KDELR. Also, the Golgi morphology was not affected in the HeLa-myc cells, as assessed by GM130, mannosidase II and TGN46 staining.

Supplementary Fig S2. shows that the transport efficiency of VSVG in HeLa-myc cells was similar to that in wild-type HeLa cells.

Supplementary Fig S3. shows that the β subunit of the COPI coatomer complex coimmunoprecipitated with the KDELR from HeLa-myc cell lysate.

  1. Supplementary Material