In Vitro Behavior of Human Adipose Tissue-Derived Stem Cells on Poly(ε-caprolactone) Film for Bone Tissue Engineering Applications

Figure 12

Representative observations acquired in LSCM of the PA42-C4 line on PCL film induced in osteogenic medium up to 35 days. Calcium deposits are stained with calcein (conventional green color) and nuclei with propidium iodide (conventional red color). 3D projection of Z-Stacks of 23 optical sections with an interval of 3 μm from each other. Objective 20x.