In Vitro Behavior of Human Adipose Tissue-Derived Stem Cells on Poly(ε-caprolactone) Film for Bone Tissue Engineering Applications

Figure 3

Multipotency evaluation of the PA42-C4 cell line. (a, b, c) Representative images of Oil Red O staining at time 0 and after 10 and 20 days of adipogenic induction; the intracellular lipids are in red and nuclei counterstained with Mayer’s acid hemalum are in blue. Images acquired in brightfield microscopy. Objective 40x. (d, e, f) Representative images of Fast Blue BB staining at time 0 and after 10 and 20 days of osteogenic induction; ALP+ cells are in blue and nuclei counterstained with propidium iodide are in fluorescent red. Images acquired in brightfield microscopy for ALP+ cells and in epifluorescence for nuclei. Objective 40x. (g, h, i) Representative images of Alizarin Red S staining at time 0 and after 20 and 30 days of osteogenic induction; mineralized calcium deposits are in red. Images acquired in phase contrast microscopy. Objective 40x.

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