Effects of a Particular Heptapeptide on the IFN-α-Sensitive CML Cells

Figure 1

Construction and characterization of the plasmid expressing KLWVIPQ. (a) Agarose gel analysis of the PCR products amplified from positive (lane 1) and negative (lane 2) plasmids. Lane M: DNA molecular weight marker. (b) Agarose gel electrophoresis of the pEGFP-KLWVIPQ plasmid without (lane 1) and with (lane 2) the digestions of XhoI and AgeI. (c) Sequencing results of the recombinant plasmid pEGFP-KLWVIPQ. (d) Agarose gel analysis of the PCR products amplified from the DNA purified from KT-1/A3 (lanes 1–3) and KT-1/A3R (lanes 4–6) cells under different transfection conditions. Lanes 1 and 4: without transfection, lanes 2 and 5: transfected with pEGFP-KLWVIPQ, and lanes 3 and 6: transfected with the empty vector pEGFP-N1.

(a)

(b)

(c)

(d)