CRISPR/CAS9-Mediated Genome Editing of miRNA-155 Inhibits Proinflammatory Cytokine Production by RAW264.7 Cells

Figure 3

Cytokine production profiles of miR-155 GKO clones. RAW264.7 or indicated clones were stimulated with 100 ng/mL LPS ((a) and (b)) or LPS + IFN-γ (100 u) ((c) and (d)) for 48 hours. Supernatants were collected for ELISA analyses. . (e) cells were treated with RANKL at indicated concentrations. TRAP activity from each well was measured as described in Section 2.  . (f) TRAP-positive multinucleated cells (MNC) were counted and plotted. . Inset shows representative images of wild type RAW264.7 cells (top panel) or D6 clone (bottom panel) which have differentiated into TRAP-positive osteoclasts after RANKL stimulation (100 ng/mL).

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