Luminex and Other Multiplex High Throughput Technologies for the Identification of, and Host Response to, Environmental Triggers of Type 1 Diabetes

Table 1

Characteristics of currently available array based high throughput technologies.

Technology/manufacturer	Maximum number of analytes	Maximum number of samples	Volume of sample	Dynamic range
Luminex/Luminexcorp	500	96 or 384	1–5 µL	>4.5 logs
SimOa/Quanterix	10	384	1–10 µL	>4 logs
Flow Cytomix/Afffymetrix	20	96	25 µL	—
Cytometric Bead assay/BD biosciences	30	96	25–50 µL	—
Barcoded Magnetic Beads/Applied Biocode	128	96	1–5 µL	5 logs
Antibody arrays/Quansys Bioscience^*	25	96	30–50 µL	—
Antibody arrays/Meso Scale discovery^*	10	96 or 384	30–50 µL	>4 logs
Antigen Arrays/Thermo Scientific	10	96	40 µL	—

Detection is based on chemiluminescence or electrochemiluminescence.