MiR-183 Regulates ITGB1P Expression and Promotes Invasion of Endometrial Stromal Cells

Figure 3

ITGB1, but not AMIGO2, is the direct target of miR-183. (a) The binding site of miR-183 in the 3′UTR of ITGB1 (top) and AMIGO2 (bottom) 3′UTR, along with the mutant construct in the predicted binding region. (b) MiR-183, but not AMIGO2, directly targets ITBG1. Endometrial stromal cells were cotransfected with wild-type reporter containing the ITBG1/AMIGO2 3′UTR or mutant ITBG1/AMIGO2 3′UTR plus miR-183 or negative control using Invitrogen Lipofectamine 2000 reagents. Luminescence was measured after 24 hours of transfection. Reporter plasmid expressing ITGB1 mRNA that contains sequences potentially targeted by miR-183 displayed a significantly decreased luciferase activity in cells transfected with miR-183 (), whereas luciferase activity of reporter plasmid containing mutant sequence of ITGB1 was not changed following forced expression of miR-183. when compared to the negative control. Error bars represent ± SEM.

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