Potential Peripartum Markers of Infectious-Inflammatory Complications in Spontaneous Preterm Birth

Figure 1

Sample processing for the exploratory iTRAQ analysis. Representative MIAC and HCA-positive and negative samples were created and processed as two duplicates. Samples were depleted from 14 high-abundance proteins and digested. Resulting peptides were iTRAQ labeled, combined, and processed according to the CysTRAQ protocol. Cysteinyl and noncysteinyl iTRAQ peptides were further fractionated using high pH reversed-phase chromatography. Eventually, each fraction was analyzed using LC-MALDI-MS/MS.