Potential Peripartum Markers of Infectious-Inflammatory Complications in Spontaneous Preterm Birth

Figure 2

Sample processing for the LC-SRM verification. Each sample was subjected to immunoaffinity depletion of 14 high-abundance proteins and digested by trypsin. Portion of each sample was used to create a global internal standard (GIS). GIS peptides were dimethylated by formaldehyde-d₂ whereas peptides in each sample were labeled by formaldehyde. GIS was spiked in each individual sample prior to LC-SRM analysis.