DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells

<table class="figure-group"><tr class="fig-image" id="a"><td><object data="https://static.hindawi.com/articles/bmri/volume-2015/346134/figures/346134.fig.007a.svgz" name="346134.fig.007a" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(a) </b>Methylases treated pL/CMV/GFP (positive control)</td></tr><tr class="fig-image" id="b"><td><object data="https://static.hindawi.com/articles/bmri/volume-2015/346134/figures/346134.fig.007b.svgz" name="346134.fig.007b" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(b) </b>CMV promoter from HPCs harvested at day 2 after transduction</td></tr><tr class="fig-image" id="c"><td><object data="https://static.hindawi.com/articles/bmri/volume-2015/346134/figures/346134.fig.007c.svgz" name="346134.fig.007c" type="image/svg+xml"></object></td></tr><tr class="fig-caption"><td><b>(c) </b>CMV promoter from HPCs harvested at day 7 after transduction</td></tr></table>

<div>Bisulfite genomic sequencing of CpG in the methylated CMV promoter region. (a) Methylase treated pL/CMV/GFP (positive control), (b) CMV promoter from HPCs harvested at day of gene expression (day 2 after transduction), and (c) CMV promoter from HPCs harvested at day of gene silencing (day 7 after transduction). One circle corresponds to one cytosine in CpG. The total number of cytosine in the CMV promoter is 36. The filled circle corresponds to methylated CpG and the open circle corresponds to unmethylated CpG.</div>

BioMed Research International

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Figure 7

Figure 7: DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells 