Lunasin Inhibits Cell Proliferation via Apoptosis and Reduces the Production of Proinflammatory Cytokines in Cultured Rheumatoid Arthritis Synovial Fibroblasts

Figure 4

Effect of lunasin on the production of proinflammatory cytokines and MMPs. Synovial fibroblasts in the presence of IL-1β induction were treated with lunasin at various concentrations (0, 10, 50, 100, and 200 μM) for 72 h in serum-free DMEM medium. After treatments, the cell-free medium and RA synovial fibroblasts were collected to evaluate the secretion of IL-6 (a), IL-8 (b), MMP-1 (c), and MMP-3 (d) by their corresponding ELISA kits, respectively. (e) The gene expression of IL-6, IL-8, MMP-1, and MMP-3 was examined by RT-PCR. Data were expressed as mean ± SD for three independent experiments by GraphPad Prism Software Version 5.0. was considered a significant difference when compared with the control.

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