The Differential Proteome of the Probiotic Lactobacillus acidophilus NCFM Grown on the Potential Prebiotic Cellobiose Shows Upregulation of Two β-Glycoside Hydrolases
Table 1
Differentially abundant proteins of Lactobacillus acidophilus NCFM grown on cellobiose compared to growth on glucose shown for selected proteins having a role in carbohydrate metabolism. Differential abundance was based on Progenesis SameSpots analyses of 2D images (≥ 1.5-fold spot volume ratio change; ANOVA and false discovery rate ). A Mascot score of ≥ 80 () was used to confirm proteins identified by peptide mass fingerprint and should have a minimum of six matched peptides. Proteins are listed according to their fold change. All identified proteins including information regarding score, ANOVA, sequence coverage, peptide search and identification, MW, and pI are available in supplementary material.
Spot number
Fold change
Accession number
Protein name
Localizationa
Functional roleb
*102
−7.8
gi∣58337790
Two-component system regulator
C
RF
16
+7.4
gi∣58337043
Phospho-beta-galactosidase II
C
EM
18
+7.0
gi∣58337043
Phospho-beta-galactosidase II
C
EM
92
−3.6
gi∣58337251
2′,3′-Cyclic-nucleotide 2′-phosphodiesterase
E
PPNN
13
+3.5
gi∣58337008
Phosphoglucomutase
C
EM
60
+2.6
gi∣58337021
Triosephosphate isomerase
C
EM
37
−2.5
gi∣58337019
Glyceraldehyde-3P dehydrogenase
C
EM
17
+2.4
gi∣58337186
Phospho-beta-glucosidase
C
EM
47
+2.4
gi∣58336768
Catabolite control protein A
C
RF
122
+2.2
gi∣58337323
Putative surface layer protein
CW
CE
123
+2.0
gi∣58337019
Glyceraldehyde-3P dehydrogenase
C
EM
99
−1.9
gi∣58337088
F0F1 ATP synthase subunit alpha
C
EM
59
−1.8
gi∣58337021
Triosephosphate isomerase
C
EM
29
−1.7
gi∣58337020
Phosphoglycerate kinase
C
EM
106
−1.7
gi∣58337021
Triosephosphate isomerase
C
EM
28
−1.6
gi∣58337020
Phosphoglycerate kinase
C
EM
30
−1.6
gi∣58337020
Phosphoglycerate kinase
C
EM
31
−1.6
gi∣58337020
Phosphoglycerate kinase
C
EM
38
−1.6
gi∣58337019
Glyceraldehyde-3P dehydrogenase
C
EM
41
−1.6
gi∣58337089
F0F1 ATP synthase subunit gamma
C
EM
36
+1.5
gi∣58337019
Glyceraldehyde-3P dehydrogenase
C
EM
70
−1.5
gi∣58337019
Glyceraldehyde-3P dehydrogenase
C
EM
107
−1.5
gi∣58337021
Triosephosphate isomerase
C
EM
Localization: C, cytoplasmic; CW, cell wall; E, extracellular. bFunctional role: CE, cell envelope; EM, energy metabolism; PPNN, purines, pyrimidines, nucleosides, and nucleotides; RF, regulatory functions. *Protein identification by MS/MS was confirmed by a Mascot score of ≥ 40 () for each peptide.