Identification and Biological Characterization of Leishmania (Viannia) guyanensis Isolated from a Patient with Tegumentary Leishmaniasis in Goiás, a Nonendemic Area for This Species in Brazil

Figure 5

Photomicrographs of nonulcerated lesions obtained from wild-type C57BL/6 and BALB/c mice infected with L. (V.) braziliensis WSS5 or L. (V.) guyanensis PLR6. Mice were inoculated with 5 × 10⁶ parasites at stationary phase of growth, and 11 weeks after infection the histopathology of footpad lesions was evaluated after H&E staining. (a) C57BL/6 WT mouse infected with L. (V.) braziliensis WSS5, (b) C57BL/6 WT mouse infected with L. (V.) guyanensis PLR6, (c) BALB/c mouse infected with L. (V.) braziliensis WSS5 showing inflammatory infiltration in deep dermis, (d) BALB/c mouse infected with L. (V.) guyanensis PLR6 showing inflammatory infiltrate in deep dermis, (e) BALB/c mouse infected with L. (V.) braziliensis WSS5 (horizontal black arrow indicates the parasite; vertical black arrow indicates the infiltrate of deep dermis in (c) that contains macrophages and parasites), and (f) BALB/c mouse infected with L. (V.) guyanensis PLR6 (vertical black arrow indicates the mononuclear cell infiltrate in deep dermis in (d) that contains vacuolated macrophages without intact parasites). Areas of hypertrophic scar formation are indicated by the circles in (a) and (d).