Dose Response of Endotoxin on Hepatocyte and Muscle Mitochondrial Respiration In Vitro

Figure 3

Measurements of total free radical ROS and RNS levels in supernatants of intact cells. Total reactive oxygen species (ROS) and reactive nitrogen species (RNS) were determined after reaction with DCFH to DCF (2^′,7^′-dichlorodihydrofluorescein) in supernatants of LPS treated and control cells ( each). The fluorescence of DCF is proportional to the content of ROS and RNS in the cell culture medium. DCF was expressed per pmol DCF/μg cellular protein. Data are represented as box and whiskers (Tukey). Groups were compared using Mann-Whitney test. ^∗∗.