Evaluation of Synergetic Anticancer Activity of Berberine and Curcumin on Different Models of A549, Hep-G2, MCF-7, Jurkat, and K562 Cell Lines

Table 2

Preparation of initial stock concentration of test items.

S. number	Test item name	Initial stock concentration in mg/mL (highest soluble conc. in DMSO)	Volume of DMSO initial stock in mL	Volume of DMEM medium in mL	Final stock in DMEM in mg/mL (1st concentration)
1	Berberine + Curcumin (BECU)	250	0.025	4.975	1.25
2	Curcumin (CU)	250	0.025	4.975	1.25
3	Berberine (BE)	100	0.025	4.975	0.5

From the final stock different concentrations of the final working stocks five concentrations were prepared in DMEM medium by 4-fold and 3-fold serial dilutions as specified in Table 3. Diluted stocks were used for the study.