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BioMed Research International
Volume 2015, Article ID 356401, 7 pages
http://dx.doi.org/10.1155/2015/356401
Research Article

Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method

1Department of Biotechnology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand
2Mahidol University-Osaka University Collaborative Research Center for Bioscience and Biotechnology (MU-OU:CRC), Faculty of Science, Mahidol University, Rama VI Road, Bangkok 10400, Thailand

Received 18 December 2014; Revised 9 April 2015; Accepted 1 May 2015

Academic Editor: Pierre Colin

Copyright © 2015 Mevaree Srisawat and Watanalai Panbangred. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

The supplementary materials include 1 Table and 5 Figures. Table S1 describes the name list of specificity test for stn detection by LAMP of 102 and 57 strains of Salmonella and non-Salmonella bacteria. The 5 Supplementary Figures show the nucleotide sequence of stn and primers annealing sites (Figure S1), Phylogenetic tree of stn sequences of various bacterial strains of the family Enterobacteriaceae (Figure S2). In Figures S3, S4 and S5 show electrophoresis patterns of LAMP amplified products with and without loop primers, specificity for stn among various strains of Enterobacteriaceae and sensitivity of stn LAMP assays using purified DNA or DNA from boiled cells, respectively.

  1. Supplementary Material