Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion Stem/Progenitor Cells

Figure 3

Induction of differentiation in spiral ganglion spheres. Secondary spheres were cultured in adherent conditions with the presence of 10% FBS in DMEM/F12 medium for 96 h. Immunocytochemistry reveals that some of them were found to differentiate into glial-like cells by expressing GFAP (red). Concurrently some sphere-forming cells differentiated into neural cells by expressing neuron-specific βIII tubulin (Tuj1, green). A small population of cells was immunostained by both GFAP and Tuj1 (merge). Scale bar = 100 μm.