Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion Stem/Progenitor Cells

Figure 6

Isolation of an SP cell population was conducted on SPCs following different oxygen tension exposures for 4 days. Cells were stained with Hoechst 33342 dye either alone or in the presence of FTC and analyzed by flow cytometry. SP cells were gated and are shown as the percentage of total SPCs when cultured at 1%, 5%, or 20% O₂. Hoechst dye efflux was markedly diminished in FTC-treated SPCs.

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