Hypoxia Induces a Metabolic Shift and Enhances the Stemness and Expansion of Cochlear Spiral Ganglion Stem/Progenitor Cells

Figure 7

Cochlear spiral ganglion SPCs cultured in hypoxia conditions undergo a metabolic switch from oxidative phosphorylation to glycolysis. Real-time measurements (mean ± SEM, ) of (a) the mitochondrial OCR (pMols/min) and (b) ECAR (mpH/min) of SPCs cultured in normoxia or hypoxia conditions were measured under basal condition and in response to the indicated mitochondrial inhibitors. The OCR was lower in hypoxia condition. (c) The basal OCR and ECAR values of normoxia- and hypoxia-cultured SPCs were plotted to illustrate the difference in cellular bioenergetics (mean ± SEM, ). (d) OCR to ECAR ratios measured by the XF24 extracellular flux analyzer (mean ± SEM, ) show a significant decrease in SPCs exposed to hypoxia, indicating a metabolic transition from mitochondrial oxidative phosphorylation to glycolysis. indicates ; FCCP: carbonyl cyanide p-trifluoromethoxyphenylhydrazone; AA: antimycin A.

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