Polymorphisms of NFκB1 and IκBα and Their Synergistic Effect on Nasopharyngeal Carcinoma Susceptibility
Table 1
Distribution of genotypes of NFκB/IκBα and associations with the risk of NPC.
Genotypes/alleles
Discovery set
Validation set
Merged set
Case (%)
Controlsa (%)
Adjusted OR (95% CI)b
Case (%)
Controlsa (%)
Adjusted OR (95% CI)b
Case (%)
Controlsa (%)
Adjusted OR (95% CI)b
Total number of subjects
906
1072
684
907
1590
1979
Total number of alleles
1812
2144
1368
1814
3180
3958
NFκB1:
rs28362491 del/ins
del/del
152 (16.8)
224 (20.9)
1.00 (ref.)
117 (17.1)
195 (21.5)
1.00 (ref.)
269 (16.9)
419 (21.2)
1.00 (ref.)
del/ins
438 (48.3)
512 (47.8)
1.22 (0.94–1.56)
331 (48.4)
438 (48.3)
1.25 (0.95–1.64)
769 (49.4)
950 (48.0)
1.24 (1.03–1.49)
ins/ins
316 (34.9)
336 (31.3)
1.39 (1.06–1.82)
236 (34.5)
274 (30.2)
1.42 (1.06–1.90)
552 (34.7)
610 (30.8)
1.41 (1.16–1.71)
Trend test value
0.012
0.010
0.001
Dominant modelc
ins/ins + del/ins versus del/del
754 (83.2)
848 (79.1)
1.28 (1.01–1.63)
567 (82.9)
712 (78.5)
1.31 (1.02–1.70)
1321 (83.1)
1560 (78.8)
1.30 (1.09–1.55)
NFκB2:
rs12769316 G>A
GG
485 (53.5)
599 (55.9)
1.00 (ref.)
AG
347 (38.3)
391 (36.5)
1.06 (0.87–1.29)
AA
74 (8.2)
82 (7.6)
1.13 (0.80–1.60)
Trend test value
0.320
IκBα:
rs2233406 C>T
CC
701 (77.4)
813 (75.8)
1.00 (ref.)
TC
188 (20.7)
244 (22.8)
0.90 (0.72–1.13)
TT
17 (1.9)
15 (1.4)
1.54 (0.74–3.19)
Trend test value
0.618
IκBα:
rs696 G>A
GG
256 (28.3)
317 (29.6)
1.00 (ref.)
182 (26.6)
255 (28.1)
1.00 (ref.)
438 (27.5)
572 (28.9)
1.00 (ref.)
AG
415 (45.8)
540 (50.4)
0.94 (0.76–1.17)
318 (46.5)
463 (51.1)
0.94 (0.74–1.20)
733 (46.1)
1003 (50.7)
0.94 (0.80–1.11)
AA
235 (25.9)
215 (20.1)
1.40 (1.08–1.82)
184 (26.9)
189 (20.8)
1.33 (1.01–1.76)
419 (26.4)
404 (20.4)
1.37 (1.13–1.65)
Trend test value
0.021
0.035
0.003
Recessive modelc
AA versus GG + AA
235 (25.9)
215 (20.1)
1.46 (1.17–1.82)
184 (26.9)
188 (20.7)
1.38 (1.09–1.75)
419 (26.4)
403 (20.3)
1.41 (1.20–1.66)
Combined NFκB1/IκBα genotypes
Number of risk genotypesd
0
105 (11.6)
183 (17.1)
1.00 (ref.)
99 (14.5)
148 (16.3)
1.00 (ref.)
204 (12.8)
331 (16.7)
1.00 (ref.)
1
613 (67.7)
715 (66.7)
1.48 (1.12–1.94)
419 (61.3)
617 (68.0)
1.05 (0.76–1.34)
1032 (64.9)
1332 (67.3)
1.25 (1.02–1.52)
2
188 (20.7)
174 (16.2)
1.93 (1.39–2.69)
166 (24.3)
142 (15.7)
1.71 (1.21–2.41)
354 (22.2)
316 (16.0)
1.82 (1.44–2.31)
Trend test value
3.93 ×
9.42 ×
2.98 ×
The observed genotype frequencies among the control subjects were in agreement with the Hardy-Weinberg equilibrium ( + + = 1) in the control subjects of both sets ( > 0.05 for all). bAdjusted in a logistic regression model that included age, sex, smoking status, alcohol use, family history of cancer, and dataset. cAkaike information criterion (AIC) value. dGenotype combinations of two polymorphisms in the NFκB1 and IκBα: ins variant genotypes rs28362491 (ins/del + ins/ins) and rs696AA genotype are defined as risk genotypes.
